antiSMASH 5 feature list#

Rule-based detection of BGCs#

BGC type
Aminocoumarins
Aminoglycosides / aminocyclitols
Aryl polyenes
atypical NRPS
atypical PKS
Bacteriocins
Beta-lactams
Beta-lactones
Bottromycin
Butyrolactones
ClusterFinder fatty acid
ClusterFinder Saccharide
Cyanobactins
Cyclodipeptide synthase-based tRNA dependent peptides
(Dialkyl)resorcinols
Ectoines
fungal RiPPs
Furan
Fused (Pheganomycin-like)
Glycocin
Head-to-tail cyclised peptide
Heterocyst glycolipid synthase like PKS
Homoserine lactone
Indoles
Ladderane lipids
Lanthipeptides
Lasso peptide
Linaridin
Linear azol(in)e-containing peptides (LAPs)
Lipolanthines
Melanins
Microviridin
N-acetylglutaminylglutamine amides
N-acyl amino acids
Non-ribosomal peptides
Nucleosides
Oligosaccharide
Others
Phenazine
Phosphoglycolipids
Phosphonate
Polybrominated diphenyl ethers
Polyunsaturated fatty acids
Proteusin
Pseudopyronines
RaS RiPPs
Sactipeptide
Siderophores
Terpene
Thiopeptide
Thioamide-containing non-ribosomal peptides
Trans-AT type I PKS
Type I PKS
Type II PKS
Type III PKS

Rule-independent detection of BGCs#

Note: This function is only available in the download/locally run version

Tool
ClusterFinder

Region specific analyses#

Analysis
Domain structure of PKSs and NRPSs
NRPS: A-domain specificity prediction (NRPSpredictor)
PKS: AT specificity prediction
Identification of conserved active site motifs; stereochemistry-determining motifs
Prediction of core chemical structure (NRPS, PKS, lanthipeptides, lasso peptides, thiopeptides)
smCOG secondary metabolism-related gene family prediction
TTA codon annotation for actinomycetes
Improved prediction of gene cluster borders for fungal BGCs (CASSIS)
Protein family detection (PFAM) search on regions
GO-classifications
Resistance gene annotation

Genome-wide analyses#

Analysis
Protein family detection (PFAM) search on whole genome
Genome comparisons
ClusterBlast (identification of similar clusters in sequence genomes); link to antiSMASH database
SubClusterBlast (identification of conserved operons with known function)
KnownClusterBlast (identification of similar characterized gene clusters)
External resource
antiSMASH database
Gene ontology
MIBiG repository
NCBI BLAST+
NaPDoS
Norine

Output file formats#

Format
Genbank
HTML
JSON results file

Input file formats#

Format
FASTA (nucleotide)
FASTA + GFF3
Genbank
EMBL
Direct download via NCBI accession number